| 2015-00601-5940-17 |
Tersedia di Pustaka Kubang Putih - UIN Sjech M. Djamil Djambek Bukittinggi
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The principal aim of this thesis was to begin to determine the possible functions of a novel and B-cell specific protein, FAM129C, identified from proteomic screening of purified CLL plasma membrane fractions. Bioinformatic analysis showed that FAM129C contained a pleckstrin homology domain that probably causes the protein to be associated with the plasma membrane but lacked any other obvious domains. Using quantitative RT-PCR, I showed that FAM129C was expressed from early stages of B-cell differentiation. It was expressed at high levels in chronic lymphocytic leukaemia (CLL) and in the activated subtype of diffuse large B-cell lymphoma, where it may be a useful diagnostic marker. FAM129C was also expressed at high levels in normal B-cell populations including both na?ve pre-germinal centre and memory cell populations but interestingly was rapidly down-regulated following stimulation to proliferation. Similar down-regulation was also observed in CLL cells stimulated to proliferation in vitro. Similar down-regulation was also observed in CLL cells stimulated to proliferate in vitro. Subcellular fraction studies of FAM129C showed wide expression in many different cell fractions, but mainly in the cytoplasm. The pattern of FAM129C expression was similar to that of CXCR4 and, therefore I have speculated that there is a potential association between these two proteins in B cell development and in B cell maturation during germinal centre reaction.
The principal aim of this thesis was to begin to determine the possible functions of a novel and B-cell specific protein, FAM129C, identified from proteomic screening of purified CLL plasma membrane fractions.
The twelve chapters of this book all derive from the reflections of a prominent historian on the nature of modern Indonesian history, over a 40-year time span. A central thread running through the book is the importance of the fact that Indonesia entered the modern community of nation-states through political revolution. This revolution has often been denied or downplayed as a failure because it did not have a communist outcome like those of China and Vietnam. A much better analogy is the French revolution - a profound breaking with and discrediting of the ancien regime but without the guiding hand of a disciplined party intent on power. Like other revolutions, it demanded a huge price in violence, human suffering, and the loss of cultural traditions; like them too, it offered a glittering prize. The prize turned out not to be the freedom and equality of which the revolutionaries had dreamt, but a previously inconceivable unity enforced by a state of a completely new kind. The Faustian bargain in by which Indonesia was created in the 1940s is at the heart of this book. All the chapters save one have been revised and updated for this publication, with the injection of some additional optimism called for by post-1998 democracy. The exception is the earliest paper, from 1967, on the paroxysm of violence that punctuated Indonesia's independent history from 1965-1966. This piece has been left unchanged as a document in the early quest for understanding of those horrific events.
The twelve chapters of this book all derive from the reflections of a prominent historian on the nature of modern Indonesian history, over a 40-year time span.
