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P53 Downregulates AKR1B10 as a Negative Feedback of AMPK Activity

Aldo-keto reductase 1 B10 (AKR1B10) is a secretory protein, overexpressed in human cancer cells in different cancers, such as hepatocellular carcinoma, lung squamous carcinoma, and lung adenocarcinoma in smokers. The author's recent study revealed that AKR1B10 may play an oncogenic role in cancer development and progression, but little is known of its expression or regulation. In this study, the author reveals a part of the regulatory mechanism by investigating the role of p53 transcription factor in regulating AKR1B10 expression level in colorectal cancer cell line, HCT-8, and hepatocellular carcinoma cell line, HepG2. In addition, this study found a negative feedback loop of p53 through AKR1B10 and AMPK. This study shows that AKR1B10 promoter contains four p53 binding sites within -804bp region of the promoter which is 4kb in full length. Mutations of p53 binding sites or knockdown of p53 expression by shRNA significantly increases the basal promoter activity and the protein expression of AKR1B10. Western blot showed that overexpression of p53 protein suppresses AKR1B10 expression level. Furthermore, the author demonstrates the direct interaction of p53 protein with its four binding sites ex vivo by chromatin immunoprecipitation assay. Also, EMSA assay further confirms the binding of this transcription factor protein with the first DNA binding site. The author's Luciferase assay showed that p53 suppresses AKR1B10 promoter activity in a dose dependent manner. While AMPK is known to be a p53 stimulator, our lab found that AMPK can be induced by AKR1B10. Together these results suggest that p53 transcription factor downregulates AKR1B10 as a negative feedback of AMPK activity.

While AMPK is known to be a p53 stimulator, our lab found that AMPK can be induced by AKR1B10. Together these results suggest that p53 transcription factor downregulates AKR1B10 as a negative feedback of AMPK activity.

Laporan tahunan presiden pada ST MPR 2000

Progress report of President Abdurrahman Wahid to Indonesian People's Consultative Assembly for the year 2000.

Progress report of President Abdurrahman Wahid to Indonesian People's Consultative Assembly for the year 2000.

Enam teater

mengenal tokoh-tokoh teater modern Indonesia

Profile of six Indonesian modern theater artists.

Sebelumnya beragama Roma Katolik dan kemudian masuk Islam. Ia pernah
kuliah di Fakultas Sastra Barat, Universitas Gajah Mada sampai tingkat Sarjana
Muda. Tahun 1964 ... Secara gampang, kebudayaan menurut pengertian Rendra
adalah ikhtiar menentang determinisme alam. Namun demikian, yang dianjurkan
Rendra untuk ditentang sebetulnya bukanlah alam itu sendiri, melainkan sikap
budaya yang berkiblat pada alam, yakni kebudayaan alamiah, cara berfikir
alamiah.

Undang-Undang Pensiun

Undang-Undang no. 20 tahun 1952

Regulations on pensions for civil servants.

Se- tjara formil tugasnja Menteri Urusan Pegawai adalah untuk mengganti
tugasnja Perdana Menteri, jang disebut dalam Peraturan Pemerintah No 32
tahun 1950, dalam hal urusan kepegawaian, tapi dalam melakukan tugasnja
sehari-hari ternjata sekali, bahwa jang diutamakan adalah untuk membereskan
setjepat mungkin kepintjangan-kepintjangan dalam urusan kepegawaian jang
disebabkan oleh adanja peraturan-peraturan dobel. ^ Ditambah pula, bahwa
chusus mengenai ...

Matrilokal dan status perempuan dalam tradisi bajapuik

studi kasus tentang perempuan dalam tradisi bajapuik

Gender analysis in marriage customs and rites of Pariaman people in Minangkabau, Sumatera Barat Province

Tradisi bajapuik menggambarkan berlangsungnya proses hegemoni ideologi
patriarki dalam hubungan-hubungan sosial, yang di dalamnya tesembunyi
kepentingan komunitas laki-laki sebagai kelas superior untuk menguasai
perempuan sebagai kelas yang inferior. Terjadinya penindasan hak-hak sosial
perempuan sebagai anggota masyarakat. Kondisi hegemoni patriarki ini, secara
umum, terjelma dalam sistem adat. Ideologi patriarki yang khas matrilineal, yaitu
berlangsungnya ...